Color standardization for the immunohistochemically stained tissue section images

Refining the information about the tissue is achieved by the standardization of color in whole-slide images and digital images of tissue sections. The investigation described in this paper proposes a solution to the problems of color dissimilarity for microscope slides digitalized by various equipment. A method of color standardization using color calibration microscope slide is proposed and a piece of software is provided. The proposed method is verified on microscope slides digitalized by various digitalization tools. It is shown that the resulting colors in two images of the same fragments are more similar to each other. The prepared microscope color calibration slide is adjusted to the standardization of immunohistochemically stained tissue samples but it can be easily adjusted to other types of staining. The software is independent from color, shape and location of color spots on the calibration slide so it can be easily used with the other calibration slides designed for different needs.